This study conducted a randomized clinical trial involving 1,337 participants to assess the impact of a gratitude intervention on well-being and mental health. Participants were divided into three groups: gratitude, hassles, and neutral events. The gratitude group practiced daily gratitude listing for 14 days. Results showed that the gratitude intervention increased positive affect, happiness, and life satisfaction while reducing negative affect and depressive symptoms compared to control groups. Similar changes were observed between the gratitude and neutral events groups. The study acknowledged limitations, including a high dropout rate for online interventions, and suggested a further investigation into online intervention characteristics.